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KMID : 0363620160370010021
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Journal of Korean Oriental Medicine
2016 Volume.37 No. 1 p.21 ~ p.33
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Comparative study on stability and efficacy of Banhasasim-tang decoction depending on the preservation temperature and periods
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Jin Seong-Eun
Kim Ohn-Soon
Seo Chang-Seob
Shin Hyeun-Kyoo
Jeong Soo-Jin
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Abstract
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Objectives: Banhasasim-tang (BHSST) has been used for the treatment of the digestive and gastric diseases in Korea.
This study aimed to investigate the stability and biological activities of BHSST decoction depending on the
preservation temperature and periods.
Methods: BHSST decoction was preserved at room temperatures (R/T, 23±1℃) or refrigeration (4℃) for 0, 30, 60
and 90 days. To evaluate the stability of BHSST decoction, pH and sugar content were estimated. In addition,
high-performance liquid chromatography (HPLC) analysis was performed to determine marker compounds of BHSST
decoction. To evaluate anti-inflammatory effect, nitric oxide (NO) and prostaglandin E2 (PGE2) productions were
measured in LPS-stimulated RAW 264.7 macrophages. Antioxidant activity was examined using the assays for
3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) and 1-1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities.
Results: There was no change in pH and sugar content depending on the preservation temperature and periods of
BHSST decoction. Among the major components of BHSST, contents of liquiritin, baicalein and wogonin was
reduced time-dependently both at R/T and 4℃. Inhibitory effects of BHSST decoction on NO and PGE2 productions
were slightly decreased in a time-dependent manner by 90 days of preservation. In addition, BHSST decoction
maintained ABTS and DPPH radical scavenging activities by 60 days while significantly reducing the activities in
90 days of preservation at R/T. By contrast, BHSST decoction had no significant change of ABTS and DPPH radical
scavenging activities by 90 days at 4℃.
Conclusions: Our results suggest that the stability and efficacy of BHSST decoction are maintained for 60 days at
4℃ rather than R/T.
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KEYWORD
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Banhasasim-tang decoction, preservation temperature, preservation period: anti-inflammation, anti-oxidation
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